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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- HPA008900 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA008900, RRID:AB_1845547
- Product name
- Anti-CHAMP1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human CHAMP1, Gene description: chromosome alignment maintaining phosphoprotein 1, Alternative Gene Names: C13orf8, CAMP, CHAMP, ZNF828, Validated applications: IHC, ICC, Uniprot ID: Q96JM3, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Chromosome alignment‐maintaining phosphoprotein CHAMP1 plays a role in cell survival through regulating Mcl‐1 expression
Dynamic feature of mitotic arrest deficient 2–like protein 2 (MAD2L2) and structural basis for its interaction with chromosome alignment–maintaining phosphoprotein (CAMP)
Hino M, Iemura K, Ikeda M, Itoh G, Tanaka K
Cancer Science 2021;112(9):3711-3721
Cancer Science 2021;112(9):3711-3721
Dynamic feature of mitotic arrest deficient 2–like protein 2 (MAD2L2) and structural basis for its interaction with chromosome alignment–maintaining phosphoprotein (CAMP)
Hara K, Taharazako S, Ikeda M, Fujita H, Mikami Y, Kikuchi S, Hishiki A, Yokoyama H, Ishikawa Y, Kanno S, Tanaka K, Hashimoto H
Journal of Biological Chemistry 2017;292(43):17658-17667
Journal of Biological Chemistry 2017;292(43):17658-17667
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Enhanced validation
- Submitted by
- 55af80e3e0991
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Confocal images of immunofluorescently stained human U-2 OS cells.The protein CHAMP1 is shown in green and the microtubules in red. The image to the left show cells transfected with control siRNA and the image to the right show cells where CHAMP1 has been downregulated with specific siRNA.
- Sample type
- U-2 OS cells
- Primary Ab dilution
- 1:53
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:800
- Knockdown/Genetic Approaches Application
- Immunocytochemistry
