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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [2]
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- Product number
- MAB5757 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Placental Lactogen/CSH1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Placental Lactogen/CSH1 in direct ELISAs and Western blot. In direct ELISAs, no cross-reactivity with recombinant human Growth Hormone is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P01243- Isotype
- IgG
- Antibody clone number
- 658230
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Placental lactogen is expressed but is not translated into protein in breast cancer.
Tuttle TR, Hugo ER, Tong WS, Ben-Jonathan N
PloS one 2014;9(1):e87325
PloS one 2014;9(1):e87325
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Placental Lactogen/CSH1 in Human Placenta. Placental Lactogen/CSH1 was detected in immersion fixed paraffin-embedded sections of human placenta using Human Placental Lactogen/CSH1 Monoclonal Antibody (Catalog # MAB5757) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in syncytiotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Placental Lactogen/CSH1 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF Membrane was probed with 2 µg/mL of Human Placental Lactogen/CSH1 Monoclonal Antibody (Catalog # MAB5757) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Placental Lactogen/CSH1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
