NBP2-22246

antibody from Novus Biologicals

Targeting: FXR1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-22246

Provider

Novus Biologicals

Product name

Rabbit Polyclonal FXR1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22246 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from mouse NIH3T3 (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-FXR1 antibody used for WB at 0.4 ug/ml. Detection: chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: FXR1 Antibody [NBP2-22246] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22246 used for IP at 6 ug/mg lysate. FXR1 was also immunoprecipitated by rabbit anti-FXR1 antibody. For blotting immunoprecipitated FXR1, NBP2-22246 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: FXR1 Antibody [NBP2-22246] - Adult mouse heart. A: 30 ug whole cell lysate. B: IgG-IP. C: Fxr1-antibody-IP. 10% SDS-PAGE, antibody diluted 1:2000 in WB assay and 3ug/mg in IP assay. This image was submitted via customer review.