Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 850402 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#850402, RRID:AB_2715875
- Product name
- Purified anti-Rab7A
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rat
- Conjugate
- Unconjugated
- Antibody clone number
- W16034A
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Western blot of anti-Rab7A antibody (clone W16034A) and isotype-matched IgG2A control. Blots were incubated with 2µg/ml (left) or IgG2A isotype control antibody (right) overnight at 4°C, followed by the incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system. M: Molecular weight marker; brain lysates: 20 µg; recombinant proteins: 10 ng. The anti-α-tubulin antibody was used as a loading control. Note that the band in the lane loaded with GST-Rab7A proteins in the blot probed with α-tubulin antibody (bottom left) is the remaining signal from the blot probed with anti-Rab7A antibody. Anti-GST antibody was used to confirm that GST-tagged recombinant proteins were loaded in the indicated lanes (data not shown).
- Conjugate
- Unconjugated