PA5-109239

antibody from Invitrogen Antibodies

Targeting: RAB7A CMT2B, RAB7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109239

Provider

Invitrogen Antibodies

Product name

RAB7A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: MCF-7, A431, SiHa, rat brain tissue, human tonsil tissue, human liver tissue, human colon cancer tissue, mouse colon tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RAB7 in MCF-7 cell lysate. Samples were incubated with RAB7 polyclonal antibody (Product # PA5-109239), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RAB7 in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with RAB7 polyclonal antibody (Product # PA5-109239), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RAB7 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with RAB7 polyclonal antibody (Product # PA5-109239), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RAB7 in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with RAB7 polyclonal antibody (Product # PA5-109239), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RAB7 in paraffin-embedded rat brain tissue. Samples were incubated with RAB7 polyclonal antibody (Product # PA5-109239), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RAB7 in paraffin-embedded mouse colon tissue. Samples were incubated with RAB7 polyclonal antibody (Product # PA5-109239), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RAB7 in paraffin-embedded human tonsil tissue. Samples were incubated with RAB7 polyclonal antibody (Product # PA5-109239), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RAB7 in paraffin-embedded human liver tissue. Samples were incubated with RAB7 polyclonal antibody (Product # PA5-109239), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RAB7 in paraffin-embedded human colon cancer tissue. Samples were incubated with RAB7 polyclonal antibody (Product # PA5-109239), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of RAB7 in A431 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with RAB7 polyclonal antibody (Product # PA5-109239) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.