LS-C148218

antibody from LSBio

Targeting: BAG3

Western blot (Enhanced validation)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C148218

Provider

LSBio

Product name

BAG3 / BAG-3 Antibody (C-Terminus) LS-C148218

Antibody type

Polyclonal

Description

Antiserum

Reactivity

Human, Mouse

Host

Rabbit

Storage

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of BAG-3 using Polyclonal Antibody to BAG-3 at 1:2000. Tissue lysates, normalized for total protein (20 ug/lane), were from a 4 week old male mouse. BAG-3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Frozen mouse muscle tissue section stained for BAG-3 expression using Polyclonal Antibody to BAG-3 at 1:2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG-3 localizes with Z-disk proteins.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of formalin-fixed paraffin-embedded human pancreas tissue sections using Polyclonal Antibody to BAG-3 at 1:2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-Eosin counterstain.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Frozen mouse muscle tissue section stained for BAG-3 expression using Polyclonal Antibody to BAG-3 at 1:2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG-3 localizes with Z-disk proteins.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of formalin-fixed paraffin-embedded human pancreas tissue sections using Polyclonal Antibody to BAG-3 at 1:2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-Eosin counterstain.