Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 10599-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BAG3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.48 mg/mL
- Storage
- -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Mouse uterus tissue were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- HeLa cells were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- DU 145 cells were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- K-562 cells were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Rat brain tissue were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
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- Invitrogen Antibodies (provider)
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- Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using (Product # 10599-1-AP) (BAG3 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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- Invitrogen Antibodies (provider)
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- Immunofluorescent analysis of Hela cells, using BAG3 antibody 10599-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
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- Invitrogen Antibodies (provider)
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- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 10599-1-AP ( BAG3 Antibody) at dilution of 1:50 (under 10x lens).
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- Invitrogen Antibodies (provider)
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- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 10599-1-AP ( BAG3 Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- IP result of anti-BAG3 (IP:10599-1-AP, 4ug; Detection:10599-1-AP 1:1000) with K-562 cells lysate 11000ug.