Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 10-7540-25 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-Trim29 Antibody
- Antibody type
- Monoclonal
- Description
- TRIM29 (Tripartite motif-containing 29) is a member of the tripartite motif (TRIM) family of transcription factors, characterized by the conserved RING finger, B-box, and coiled-coil domains. The TRIM family has been implicated in a variety of physiologic processes including development, apoptosis and epithelial-mesenchymal transition (EMT). Depending on a tumor types, TRIM29 also may function as an oncogene or a tumor suppressor. As an oncogene, it facilitates tumor cell proliferation and invasion through stabilization of Beta-catenin. The mechanisms may also involve deactivation of p53 activity and promoting cell survival by inhibiting proapoptotic genes regulated by p53. As a histone binding protein TRIM29 is responsible for DDR (DNA damage response). It functions as a scaffold protein to assemble DNA repair proteins into chromatin followed by efficient activation of DDR. TRIM29 binds to modified histone H3 and H4 tails in the context of nucleosomes. Furthermore, chromatin binding of TRIM29 is required for the phosphorylation of H2AX nucleosomes and cell viability in response to ionizing radiation.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant Trim29
protein (amino acids 2-291) was us
ed as the immunogen for this antibo
dy.- Isotype
- IgG
- Antibody clone number
- ABM43D2
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of Trim29. Anti- Trim29 antibody (Clone: ABM43D2) was tested at 1 µg/ml Hela lysate.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Trim29 in human lung adenocarcinoma tissue using Trim29 antibody (Clone: ABM43D2) at 1 µg/ml.
- Protocol
- Protocol