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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP1-52000 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-52000, RRID:AB_11028953
- Product name
- Goat Polyclonal TRIM29 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
C-EKDRIKSFTTNE- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Oncogenic function of ATDC in pancreatic cancer through Wnt pathway activation and beta-catenin stabilization.
Wang L, Heidt DG, Lee CJ, Yang H, Logsdon CD, Zhang L, Fearon ER, Ljungman M, Simeone DM
Cancer cell 2009 Mar 3;15(3):207-19
Cancer cell 2009 Mar 3;15(3):207-19
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRIM29 Antibody [NBP1-52000] - (1ug/ml) staining of Peripheral Blood Mononucleocyte (A) and (0.1 ug/ml) HeLa (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TRIM29 Antibody [NBP1-52000] - (3.8ug/ml) staining of Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TRIM29 Antibody [NBP1-52000] - analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
