PA5-30488

antibody from Invitrogen Antibodies

Targeting: TRIM29 ATDC, FLJ36085

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30488

Provider

Invitrogen Antibodies

Product name

TRIM29 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431. Predicted reactivity: Mouse (86%), Rat (87%), Rhesus Monkey (98%), Bovine (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.99 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Terminal keratinocyte differentiation in vitro is associated with a stable DNA methylome.

Smits JPH, Dirks RAM, Qu J, Oortveld MAW, Brinkman AB, Zeeuwen PLJM, Schalkwijk J, Zhou H, Marks H, van den Bogaard EH
Experimental dermatology 2021 Aug;30(8):1023-1032

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The maintenance ability and Ca(2+) availability of skeletal muscle are enhanced by sildenafil.

Huang M, Lee KJ, Kim KJ, Ahn MK, Cho CH, Kim DH, Lee EH
Experimental & molecular medicine 2016 Dec 9;48(12):e278

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TRIM29 antibody immunoprecipitates TRIM29 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : 30 µg whole cell lysate/extract of TRIM29 protein expressing A431 cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of TRIM29 by 2.5 µg of TRIM29 antibody (Product # PA5-30488) 10% SDS-PAGE The immunoprecipitated TRIM29 protein was detected by TRIM29 antibody (Product # PA5-30488) diluted at 1:2,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Differentially methylated probes upon differentiation of primary keratinocytes and siRNA targeted knock-down of TRIM29. A, B, C, UCSC genome browser screenshots showing two significantly differentially methylated probes (in red) in the MethylationEPIC beadchip array. Both examples show hypomethylation on day6 in comparison with day0. The RNA sequencing genome browser tracks at the bottom of panel A and C show TRIM29 expression which in comparison with day0 is increased at day2, but declines at day4 and day7. The unannotated gene in panel B is not expressed. D, Transcriptional expression induction of TRIM29 as analysed by RT-qPCR. E, SMARTpool siRNA against TRIM29 mediated the knock-down of TRIM29 expression at day7 of keratinocyte differentiation, shown as mean gene expression for 3 primary keratinocyte donors. F, Western blot shows TRIM29 protein depletion upon siRNA treatment, in contrast to non-targeting siRNA (siCtrl). G, Subsequent to TRIM29 knock-down, gene expression of several differentiation genes ( FLG , IVL , LOR , HRNR , TGM1 , TGM3 and KRT10 ) was assessed after 7 d of differentiation. Although single genes (except for TGM3 ) are not significantly lowered, the set of genes is as follows: P-value .0002. Expression of genes associated with proliferating (basal) keratinocytes, such as KRT5 , KRT14 and MKI67 , was elevated upon TRIM29 knock-down. * P-value < .05, ** P-value < .01. *** P-value < .001. N = 5 primary keratinocyte donors (A,B,C,D) and N = 3 prim