OAAB01524

antibody from Aviva Systems Biology

Targeting: RFC5 RFC36

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

OAAB01524

Provider

Aviva Systems Biology

Product name

RFC5 Antibody

Antibody type

Polyclonal

Reactivity

Human, Rat

Host

Rabbit

Vial size

400ul

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Western blot analysis of anti-RFC5 Antibody (Center R185) in Hela cell lysates (35ug/lane). RFC5(arrow) was detected using the purified Pab.

Sample type

Hela cell line lysates

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Western blot analysis of anti-RFC5 Antibody (Center R185) in rat PASMC cell lysates (35ug/lane). RFC5(arrow) was detected using the purified Pab( Provided by Hirotaka Ata).

Sample type

rat PASMC cell line lysates

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Western blot analysis of RFC5 (arrow) using rabbit polyclonal RFC5 Antibody (Center R185) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RFC5 gene.

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol

Flow cytometry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

RFC5 Antibody (Center R185) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Sample type

Hela cells

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol