Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Flow cytometry [1]
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Validation data
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- Product number
- OAAB01524 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- RFC5 Antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of anti-RFC5 Antibody (Center R185) in Hela cell lysates (35ug/lane). RFC5(arrow) was detected using the purified Pab.
- Sample type
- Hela cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of anti-RFC5 Antibody (Center R185) in rat PASMC cell lysates (35ug/lane). RFC5(arrow) was detected using the purified Pab( Provided by Hirotaka Ata).
- Sample type
- rat PASMC cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of RFC5 (arrow) using rabbit polyclonal RFC5 Antibody (Center R185) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RFC5 gene.
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- RFC5 Antibody (Center R185) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Sample type
- Hela cells
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol