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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-29876 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2, mouse liver, rat liver.
- Concentration
- 1 mg/mL
Submitted references PLS3 Overexpression Delays Ataxia in Chp1 Mutant Mice.
An orthogonal proteomic survey uncovers novel Zika virus host factors.
Janzen E, Wolff L, Mendoza-Ferreira N, Hupperich K, Delle Vedove A, Hosseinibarkooie S, Kye MJ, Wirth B
Frontiers in neuroscience 2019;13:993
Frontiers in neuroscience 2019;13:993
An orthogonal proteomic survey uncovers novel Zika virus host factors.
Scaturro P, Stukalov A, Haas DA, Cortese M, Draganova K, Płaszczyca A, Bartenschlager R, Götz M, Pichlmair A
Nature 2018 Sep;561(7722):253-257
Nature 2018 Sep;561(7722):253-257
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CHP1 Polyclonal Antibody (Product # PA5-29876) and a 22 kDa band corresponding to CHP-1 was observed in all the tested cell lines. Whole cell lysates (30 µg lysate) of HeLa (Lane 1), PANC-1 (Lane 2), OVCAR-3 (Lane 3), PC-3 (Lane 4), Hep G2 (Lane 5), tissue lysates (30 µg lysates) of Mouse lung (Lane 6) and Rat Lung (Lane 7) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CHP1 Polyclonal Antibody (Product # PA5-29876). Sample (30 µg of whole cell lysate). Lane A: HeLa. Lane B: HepG2. 12% SDS PAGE. CHP1 Polyclonal Antibody (Product # PA5-29876) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CHP1 Polyclonal Antibody detects CHP protein by western blot analysis. A. 50 µg mouse liver lysate/extract.12% SDS-PAGE. CHP1 Polyclonal Antibody (Product # PA5-29876) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CHP1 Polyclonal Antibody detects CHP protein by western blot analysis. A. 50 µg rat liver lysate/extract.12% SDS-PAGE. CHP1 Polyclonal Antibody (Product # PA5-29876) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of CHP1 was achieved by transfecting HeLa with CHP1 specific siRNAs (Silencer® select Products # s22235, s22236). Western blot analysis (Fig. a) was performed using whole cell extracts from the CHP1 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with CHP1 Polyclonal Antibody (Product # PA5-29876, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to CHP1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CHP1 Polyclonal Antibody (Product # PA5-29876) and a 22 kDa band corresponding to CHP-1 was observed in all the tested cell lines. Whole cell lysates (30 µg lysate) of HeLa (Lane 1), PANC-1 (Lane 2), OVCAR-3 (Lane 3), PC-3 (Lane 4), Hep G2 (Lane 5), tissue lysates (30 µg lysates) of Mouse lung (Lane 6) and Rat Lung (Lane 7) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CHP1 Polyclonal Antibody detects Calcium binding protein P22 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcium binding protein P22 protein stained by CHP1 Polyclonal Antibody (Product # PA5-29876) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Calcium binding protein P22 antibody detects Calcium binding protein P22 protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Calcium binding protein P22 antibody (Product # PA5-29876) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Calcium binding protein P22 (Product # PA5-29876) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
