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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-14947 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal engrailed homeobox 2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: engrailed homeobox 2 Antibody [NBP2-14947] - Sample (30 ug of whole cell lysate) A: MCF-7 12% SDS PAGE; antibody diluted at 1:10000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: engrailed homeobox 2 Antibody [NBP2-14947] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of EN2 protein by 2 ug of EN2 antibody 12% SDS-PAGE The immunoprecipitated EN2 protein was detected by EN2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
