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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-28953 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EN2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: MCF-7. Predicted reactivity: Mouse (84%), Rat (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EN2 Polyclonal Antibody (Product # PA5-28953). Sample (30 µg of whole cell lysate). Lane A: MCF-7. 12% SDS PAGE. EN2 Polyclonal Antibody (Product # PA5-28953) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EN2 Polyclonal Antibody immunoprecipitates EN2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of EN2 protein by 2 µg of EN2 Polyclonal Antibody (Product # PA5-28953) 12% SDS-PAGE The immunoprecipitated EN2 protein was detected by EN2 Polyclonal Antibody (Product # PA5-28953) diluted at 1:1,000.
