NBP2-22278
antibody from Novus Biologicals
Targeting: RBM33
DKFZp434D1319, DKFZp686F102, MGC20460, PRR8
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-22278 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal RBM33 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBM33 Antibody [NBP2-22278] - Whole cell lysate from Jurkat (15 and 50 ug), HeLa (50 ug), and 293T (50 ug) cells. NBP2-22278 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RBM33 Antibody [NBP2-22278] - Whole cell lysate from mouse NIH3T3 (50 ug), TCMK-1 (50 ug), and 4T1 (50 ug) cells. NBP2-22278 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RBM33 Antibody [NBP2-22278] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-RBM33 antibody NBP2-22278 used for IP at 6 ug/mg lysate. RBM33 was inefficiently immunoprecipitated by rabbit anti-RBM33 antibodies NBP2-22279 and NBP2-22280. For blotting immunoprecipitated RBM33, NBP2-22280 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.