A303-926A

antibody from Invitrogen Antibodies

Targeting: RBM33 DKFZp434D1319, DKFZp686F102, MGC20460, PRR8

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

A303-926A

Provider

Invitrogen Antibodies

Product name

RBM33 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human RBM33 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-RBM33 antibody A303-926A (lot A303-926A-1) used for IP at 6 µg/mg lysate. RBM33 was inefficiently immunoprecipitated by rabbit anti-RBM33 antibodies A303-927A and A303-928A. For blotting immunoprecipitated RBM33, A303-928A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse RBM33 by western blot. Samples: Whole cell lysate from mouse NIH3T3 (50µg), TCMK-1 (50µg), and 4T1 (50µg) cells. Antibodies: Affinity purified rabbit anti-RBM33 antibody A303-926A (lot A303-926A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human RBM33 by western blot. Samples: Whole cell lysate from Jurkat (15 and 50 µg), HeLa (50µg), and 293T (50µg) cells. Antibodies: Affinity purified rabbit anti-RBM33 antibody A303-926A (lot A303-926A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.