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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- ABIN615953 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-DCN1, Defective in Cullin Neddylation 1, Domain Containing 1 (S. Cerevisiae) (DCUN1D1) (C-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide with sequence from the C Terminus of the protein sequence according to NP_065691.2. Genename: DCUN1D1
- Description
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
- Reactivity
- Human, Mouse, Bovine
- Host
- Goat
- Antigen sequence
C-RPQIAGTKSTT- Epitope
- C-Term
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Staining of HepG2 lysate using DCUN1D1 Antibody Cat.-No AP23734PU-N at 0.5 μg/ml (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Staining of paraffin embedded Human Kidney using DCUN1D1 Antibody Cat.-No AP23734PU-N at 5 μg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
