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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Other assay [1]
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- Product number
- A305-623A-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCLN Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human NCLN by western blot. Samples: Whole cell lysate (50 µg) from LNCaP, HEK293T, HeLa, K-562, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCLN antibody (Product # A305-623A-T lot 3) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse NCLN by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCLN antibody (Product # A305-623A-T lot 3) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human NCLN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCLN antibody A305-623A-M (lot A305-623A-M-1) used for IP at 50 µl per reaction. NCLN was also immunoprecipitated by rabbit anti-NCLN antibody BL22274. For blotting immunoprecipitated NCLN, A305-623A-M was used at 1:100. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse NCLN by western blot. Samples: Whole cell lysate (15 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCLN antibody A305-623A-M (lot A305-623A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human NCLN by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NCLN antibody (Product # A305-623A-T; lot 3) used for IP at 20 µL per reaction. NCLN was also immunoprecipitated by a previous lot of this antibody (Product # A305-623A-T; lot 2) and an antibody against a different epitope of NCLN (BL22274). For blotting immunoprecipitated NCLN, Product # A305-623A-M was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.
