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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-23400 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POU3F2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- PA5-23400 targets POU3F2 in IHC (P) and WB applications and shows reactivity with Bovine, Canine, Equine, Human, mouse, and Rat samples. The PA5-23400 immunogen is synthetic peptide (254 AHHDPHSDEDTPTSDD 269) of POU3F2 protein.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POU3F2 in human Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) mouse NIH 3T3 cell lysate in absence of immunizing peptide using a POU3F2 polyclonal antibody (Product # PA5-23400) at3 µg/mL. Goat anti-rabbit Ig HRP secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of POU3F2 in human Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) mouse NIH 3T3 cell lysate in absence of immunizing peptide. Samples were incubated in POU3F2 polyclonal antibody (Product # PA5-23400) followed by a goat anti-rabbit Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
