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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB7427 - Provider product page
- Provider
- R&D Systems
- Product name
- Human POU3F2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human POU3F2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) POU5F1 or rhPOU4F3 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P20265- Isotype
- IgG
- Antibody clone number
- 761340
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human POU3F2 by Western Blot. Western blot shows lysates of A375 human melanoma cell line, Bowes human melanoma cell line, and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human POU3F2 Monoclonal Antibody (Catalog # MAB7427) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for POU3F2 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
