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LearnMA5-18075
antibody from Invitrogen Antibodies
Targeting: SYNE2
DKFZP434H2235, KIAA1011, Nesp2, Nesprin-2, NUA, NUANCE, SYNE-2
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- MA5-18075 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nesprin 2 Monoclonal Antibody (K20-478-5)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- K20-478-5
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Components of the LINC and NPC complexes coordinately target and translocate a virus into the nucleus to promote infection.
ATR is essential for preservation of cell mechanics and nuclear integrity during interstitial migration.
Spriggs CC, Cha G, Li J, Tsai B
PLoS pathogens 2022 Sep;18(9):e1010824
PLoS pathogens 2022 Sep;18(9):e1010824
ATR is essential for preservation of cell mechanics and nuclear integrity during interstitial migration.
Kidiyoor GR, Li Q, Bastianello G, Bruhn C, Giovannetti I, Mohamood A, Beznoussenko GV, Mironov A, Raab M, Piel M, Restuccia U, Matafora V, Bachi A, Barozzi S, Parazzoli D, Frittoli E, Palamidessi A, Panciera T, Piccolo S, Scita G, Maiuri P, Havas KM, Zhou ZW, Kumar A, Bartek J, Wang ZQ, Foiani M
Nature communications 2020 Sep 24;11(1):4828
Nature communications 2020 Sep 24;11(1):4828
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Nesprin 2 in paraformaldehyde-fixed HaCaT cells using a Nesprin 2 monoclonal antibody (Product # MA5-18075).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Nesprin 2 in paraformaldehyde-fixed HaCaT cells using a Nesprin 2 monoclonal antibody (Product # MA5-18075).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6 ATR interactors. a Schematic summary of ATR interactors and their relative roles at the nuclear envelope and perinuclear areas b Validation of Nesprin-2 and ATR interaction using GFP-ATR immunoprecipitation and western blot analysis (full blots of Nesprin-2 with molecular weights included in Supplementary Fig. 4h and in Source data file). c Example of ATR-Nesprin-2 interaction foci generated by proximity ligation assay (PLA) in interphase and prophase cells (scale bar = 10 mum). d Quantification of total number of foci present in interphase and prophase cells ( n = 65 for interphase and 10 for mitosis, pooled from 2 independent experiments). Graph presented as mean +- SEM. p -value calculated using two-tailed Student's t -test.
