PA5-51933

antibody from Invitrogen Antibodies

Targeting: SYNE2 DKFZP434H2235, KIAA1011, Nesp2, Nesprin-2, NUA, NUANCE, SYNE-2

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-51933

Provider

Invitrogen Antibodies

Product name

Nesprin 2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: NVLNDAYENL TRYKEAVTRA VESITSLEAI IIPYRVDVGN PEESLEMPLR KQEELESTVA RIQDLTEKLG MISSPEAKLQ LQYTLQELVS KNSAMKEAFK AQETEAE Highest antigen sequence identity to the following orthologs: Mouse - 56%, Rat - 29%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.3 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Lamin A-mediated nuclear lamina integrity is required for proper ciliogenesis.

Fan JR, You LR, Wang WJ, Huang WS, Chu CT, Chi YH, Chen HC
EMBO reports 2020 Oct 5;21(10):e49680

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of Nesprin 2 in human cell line U-251 MG shows positivity in nucleus & nuclear membrane. Samples were probed using a Nesprin 2 Polyclonal Antibody (Product # PA5-51933).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Impaired ciliogenesis by depletion of lamin A/C may partially result from nesprin 2 suppression A An equal amount of whole-cell lysates from RPE cells or those expressing shLamin A/C or luciferase (shLuc) was analyzed by the immunoblotting with antibodies as indicated. B-D RPE cells were infected with lentiviruses expressing shRNAs to nesprin 2 (shNesprine 2, clone #1, and clone #2) or luciferase (shLuc) as a control. An equal amount of whole-cell lysates was analyzed by immunoblotting with antibodies as indicated (B). The cells were serum-starved for 48 h and then stained for acetylated tubulin (white), pericentrin (green), F-actin (red), and DNA (blue). The representative images are shown (C) The left insets show the merged signals of acetylated tubulin and pericentrin. Scale bars, 20 or 2 mum (magnified images). The numbers of F-actin stress fibers within 60 mum 2 around the basal bodies (as illustrated by the circle) were measured (D, n >= 53). E, F RPE cells expressing shNesprine 2 or shLuc were serum-starved for 32 h and then treated with (+) or without (-) Cyto D for another 16 h. The percentage of the cells with cilia in the total counted cells (E, n >= 197) and the length of cilia (F, n >= 52) were measured. Data information: In D and E, values (means +- SD) are from three independent experiments. In F, values (means +- SEM) are from three independent experiments. Statistical significance of differences is assessed with Student's t -test.