A305-875A-T

antibody from Invitrogen Antibodies

Targeting: URB2 KIAA0133, NET10, NPA2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-875A-T

Provider

Invitrogen Antibodies

Product name

URB2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human URB2 by western blot. Samples: Whole cell lysate (50 µg) from RKO, K-562, MCF-7, LNCaP, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-URB2 antibody A305-875A-M (lot A305-875A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human URB2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RKO cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-URB2 antibody (Product # A305-875A-T; lot A305-875A-T-1) used for IP at 15 µL per reaction. URB2 was also immunoprecipitated by rabbit anti-URB2 antibody BL24215. For blotting immunoprecipitated URB2, Product # A305-875A-T was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.