PA3-021

antibody from Invitrogen Antibodies

Targeting: GPR32 RVDR1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA3-021

Provider

Invitrogen Antibodies

Product name

GPR32 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

IHC (P) analysis shows positive staining of GPR32 in human small intestine tissue neuroendocrine cells. WB analysis shows GPR32 in glycoprotein-enriched fractions from GPR32 overexpressing 293 cells. Additional unknown bands at ~30kD and ~130kD were also detected. While GPR32 has a theoretical MW of ~ 40kD, the observed band in a WB runs at ~60kD due to enrichment of the glycosylated receptor.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

-20°C

References

Submitted references

Passive and active inclusion of host proteins in human immunodeficiency virus type 1 gag particles during budding at the plasma membrane.

Hammarstedt M, Garoff H
Journal of virology 2004 Jun;78(11):5686-97

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Differential display implicates cyclophilin A in adult cortical plasticity.

Arckens L, Van der Gucht E, Van den Bergh G, Massie A, Leysen I, Vandenbussche E, Eysel UT, Huybrechts R, Vandesande F
The European journal of neuroscience 2003 Jul;18(1):61-75

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Interaction of the retinoblastoma gene product, RB, with cyclophilin A negatively affects cyclosporin-inhibited NFAT signaling.

Cui Y, Mirkia K, Florence Fu YH, Zhu L, Yokoyama KK, Chiu R
Journal of cellular biochemistry 2002;86(4):630-41

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The hydrophobic pocket of cyclophilin is the binding site for the human immunodeficiency virus type 1 Gag polyprotein.

Braaten D, Ansari H, Luban J
Journal of virology 1997 Mar;71(3):2107-13

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GPR32 was performed on neuroendocrine cells in human small intestine tissue. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with a GPR32 polyclonal antibody (Product # PA3-021) at a dilution of 1:3000, overnight at 4C in a humidified chamber. Tissues were washed, and detection was performed using an ABC kit composed of biotinylated goat anti-rabbit IgG, peroxidase-conjugated avidin, and 3-amino-9-ethylcarbazole (AEC) substrate in acetate buffer. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting.