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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- HPA017340 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA017340, RRID:AB_2296925
- Product name
- Anti-LEMD2
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human LEMD2, Gene description: LEM domain containing 2, Alternative Gene Names: dJ482C21.1, NET25, Validated applications: WB, IHC, ICC, Uniprot ID: Q8NC56, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references A novel role for CSA in the regulation of nuclear envelope integrity: uncovering a non-canonical function
Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase
Oligomeric CHMP7 mediates three-way ER junctions and ER-mitochondria interactions
Loss of function of the nuclear envelope protein LEMD2 causes DNA damage–dependent cardiomyopathy
Mechanisms of A-Type Lamin Targeting to Nuclear Ruptures Are Disrupted in LMNA- and BANF1-Associated Progerias
The nuclear envelope protein Net39 is essential for muscle nuclear integrity and chromatin organization
CDK1 controls CHMP7-dependent nuclear envelope reformation
LEM2 phase separation promotes ESCRT-mediated nuclear envelope reformation
SATB2‐LEMD2 interaction links nuclear shape plasticity to regulation of cognition‐related genes
BAF facilitates interphase nuclear membrane repair through recruitment of nuclear transmembrane proteins
Nuclear formation induced by DNA-conjugated beads in living fertilised mouse egg
Repair of nuclear ruptures requires barrier-to-autointegration factor
LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells
Yang D, Lai A, Davies A, Janssen A, Ellis M, Larrieu D
Life Science Alliance 2024;7(11):e202402745
Life Science Alliance 2024;7(11):e202402745
Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase
Haraguchi T, Koujin T, Shindo T, Bilir Ş, Osakada H, Nishimura K, Hirano Y, Asakawa H, Mori C, Kobayashi S, Okada Y, Chikashige Y, Fukagawa T, Shibata S, Hiraoka Y
Communications Biology 2022;5(1)
Communications Biology 2022;5(1)
Oligomeric CHMP7 mediates three-way ER junctions and ER-mitochondria interactions
Chu Q, Wang J, Du Y, Zhou T, Shi A, Xiong J, Ji W, Deng L
Cell Death & Differentiation 2022;30(1):94-110
Cell Death & Differentiation 2022;30(1):94-110
Loss of function of the nuclear envelope protein LEMD2 causes DNA damage–dependent cardiomyopathy
Caravia X, Ramirez-Martinez A, Gan P, Wang F, McAnally J, Xu L, Bassel-Duby R, Liu N, Olson E
Journal of Clinical Investigation 2022;132(22)
Journal of Clinical Investigation 2022;132(22)
Mechanisms of A-Type Lamin Targeting to Nuclear Ruptures Are Disrupted in LMNA- and BANF1-Associated Progerias
Sears R, Roux K
Cells 2022;11(5):865
Cells 2022;11(5):865
The nuclear envelope protein Net39 is essential for muscle nuclear integrity and chromatin organization
Ramirez-Martinez A, Zhang Y, Chen K, Kim J, Cenik B, McAnally J, Cai C, Shelton J, Huang J, Brennan A, Evers B, Mammen P, Xu L, Bassel-Duby R, Liu N, Olson E
Nature Communications 2021;12(1)
Nature Communications 2021;12(1)
CDK1 controls CHMP7-dependent nuclear envelope reformation
Gatta A, Olmos Y, Stoten C, Chen Q, Rosenthal P, Carlton J
eLife 2021;10
eLife 2021;10
LEM2 phase separation promotes ESCRT-mediated nuclear envelope reformation
von Appen A, LaJoie D, Johnson I, Trnka M, Pick S, Burlingame A, Ullman K, Frost A
Nature 2020;582(7810):115-118
Nature 2020;582(7810):115-118
SATB2‐LEMD2 interaction links nuclear shape plasticity to regulation of cognition‐related genes
Feurle P, Abentung A, Cera I, Wahl N, Ablinger C, Bucher M, Stefan E, Sprenger S, Teis D, Fischer A, Laighneach A, Whitton L, Morris D, Apostolova G, Dechant G
The EMBO Journal 2020;40(3)
The EMBO Journal 2020;40(3)
BAF facilitates interphase nuclear membrane repair through recruitment of nuclear transmembrane proteins
Young A, Gunn A, Hatch E, Misteli T
Molecular Biology of the Cell 2020;31(15):1551-1560
Molecular Biology of the Cell 2020;31(15):1551-1560
Nuclear formation induced by DNA-conjugated beads in living fertilised mouse egg
Suzuki Y, Bilir Ş, Hatano Y, Fukuda T, Mashiko D, Kobayashi S, Hiraoka Y, Haraguchi T, Yamagata K
Scientific Reports 2019;9(1)
Scientific Reports 2019;9(1)
Repair of nuclear ruptures requires barrier-to-autointegration factor
Halfmann C, Sears R, Katiyar A, Busselman B, Aman L, Zhang Q, O’Bryan C, Angelini T, Lele T, Roux K
Journal of Cell Biology 2019;218(7):2136-2149
Journal of Cell Biology 2019;218(7):2136-2149
LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells
Gu M, LaJoie D, Chen O, von Appen A, Ladinsky M, Redd M, Nikolova L, Bjorkman P, Sundquist W, Ullman K, Frost A
Proceedings of the National Academy of Sciences 2017;114(11)
Proceedings of the National Academy of Sciences 2017;114(11)
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear membrane.
- Sample type
- Human
