Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP1-28740 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-28740, RRID:AB_1913937
- Product name
- Rabbit Polyclonal Citron Kinase Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references MYBPH, a transcriptional target of TTF-1, inhibits ROCK1, and reduces cell motility and metastasis.
Hosono Y, Yamaguchi T, Mizutani E, Yanagisawa K, Arima C, Tomida S, Shimada Y, Hiraoka M, Kato S, Yokoi K, Suzuki M, Takahashi T
The EMBO journal 2012 Jan 18;31(2):481-93
The EMBO journal 2012 Jan 18;31(2):481-93
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: Citron Kinase Antibody [NBP1-28740] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Citron antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. Citron was also immunoprecipitated by rabbit anti-Citron antibody BL5459, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).