- HPA008346 - Atlas Antibodies SUN1 antibody

Submitted references Muscular dystrophy-associated SUN1 and SUN2 variants disrupt nuclear-cytoskeletal connections and myonuclear organization.

Meinke P, Mattioli E, Haque F, Antoku S, Columbaro M, Straatman KR, Worman HJ, Gundersen GG, Lattanzi G, Wehnert M, Shackleton S
PLoS genetics 2014 Sep;10(9):e1004605

A mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton

Horn H, Kim D, Wright G, Wong E, Stewart C, Burke B, Roux K
The Journal of Cell Biology 2013 September;202(7):1023-1039

Human telomeres are tethered to the nuclear envelope during postmitotic nuclear assembly.

Crabbe L, Cesare AJ, Kasuboski JM, Fitzpatrick JA, Karlseder J
Cell reports 2012 Dec 27;2(6):1521-9

Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of Proteomics 2012 April;75(7):2236-2251

Samp1 is functionally associated with the LINC complex and A-type lamina networks

Gudise S, Figueroa R, Lindberg R, Larsson V, Hallberg E
Journal of Cell Science 2011 May;124(12):2077-2085

Induction of a massive endoplasmic reticulum and perinuclear space expansion by expression of lamin B receptor mutants and the related sterol reductases TM7SF2 and DHCR7.

Zwerger M, Kolb T, Richter K, Karakesisoglou I, Herrmann H
Molecular biology of the cell 2010 Jan 15;21(2):354-68

Membrane Insertion of the Pleckstrin Homology Domain Variable Loop 1 Is Critical for Dynamin-catalyzed Vesicle Scission

Ramachandran R, Pucadyil T, Liu Y, Acharya S, Leonard M, Lukiyanchuk V, Schmid S
Molecular Biology of the Cell 2009 November;20(22):4630-4639

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Enhanced validation

Submitted by: 55af80e3e0991
Enhanced method: Genetic validation
Main image
Experimental details: Confocal images of immunofluorescently stained human U-2 OS cells.The protein SUN1 is shown in green. The image to the left show cells transfected with control siRNA and the image to the right show cells where SUN1 has been downregulated with specific siRNA.
Sample type: U-2 OS cells
Primary Ab dilution: 1:59
Secondary Ab: Secondary Ab
Secondary Ab dilution: 1:800
Knockdown/Genetic Approaches Application: Immunocytochemistry
KD Reagent Type: siRNA
Downregulation: <50%
KD Reagent Provider: Thermo Fisher Scientific
KD Reagent Prod no: s23629
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Prod no 2: s23630
KD Reagent Page 2: KD Reagent Page 2
KD Reagent Prod no 3: s23631
KD Reagent Page 3: KD Reagent Page 3
Antibody Lot Number: R01574
Appendix: 55b74f0b3932b.pdf

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Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunofluorescent staining of human cell line A-431 shows positivity in nuclear membrane.
Sample type: HUMAN

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human skin and bone marrow tissues using HPA008346 antibody. Corresponding SUN1 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Immunohistochemical staining of human fallopian tube, kidney, skin and testis using Anti-SUN1 antibody HPA008346 (A) shows similar protein distribution across tissues to independent antibody HPA008461 (B).
Antibody #2 product nr: HPA008461
Antibody provider: Atlas Antibodies

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Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human placenta shows strong nuclear membrane positivity in trophoblastic cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human skin shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human pancreas shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human colon using Anti-SUN1 antibody HPA008346.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human lymph node using Anti-SUN1 antibody HPA008346.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human bone marrow shows low positivity in hematopoietic cells as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human skin shows strong positivity in nuclear membrane in squamous epithelial cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human kidney shows strong positivity in nuclear membrane in cells in glomeruli.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis shows strong positivity in nuclear membrane in Leydig cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human Fallopian tube shows moderate positivity in nuclear membrane in glandular cells.
Sample type: HUMAN

HPA008346

Antibody data