HPA008346

SUN1 antibody from Atlas Antibodies

FLJ12407, KIAA0810, UNC84A

Validated within the Antibodypedia Validation Initative

Immunocytochemistry

Enhanced validation

Immunohistochemistry

Enhanced validation

Provider product page for HPA008346

Antibody Data

Product number

HPA008346

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA008346, RRID:AB_888851

Product name

Anti-SUN1

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

QLLPTVEHLQLELDQLKSELSSWRHVKTGCETVDA
VQERVDVQVREMVKLLFSEDQQGGSLEQLLQRFSS
QFVSKGDLQTMLRDLQLQILRNVTHHVSVTKQLPT
SEAVVSAVSEAGASGITEAQARAIVNS


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Muscular Dystrophy-Associated SUN1 and SUN2 Variants Disrupt Nuclear-Cytoskeletal Connections and Myonuclear Organization
Meinke P, Mattioli E, Haque F, Antoku S, Columbaro M, Straatman K, Worman H, Gundersen G, Lattanzi G, Wehnert M, Shackleton S, Cox G
PLoS Genetics 2014 September;10(9)

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A mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton
Horn H, Kim D, Wright G, Wong E, Stewart C, Burke B, Roux K
The Journal of Cell Biology 2013 September;202(7):1023-1039

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Human Telomeres Are Tethered to the Nuclear Envelope during Postmitotic Nuclear Assembly
Crabbe L, Cesare A, Kasuboski J, Fitzpatrick J, Karlseder J
Cell Reports 2012 December;2(6):1521-1529

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Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of Proteomics 2012 April;75(7):2236-2251

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Samp1 is functionally associated with the LINC complex and A-type lamina networks
Gudise S, Figueroa R, Lindberg R, Larsson V, Hallberg E
Journal of Cell Science 2011 May;124(12):2077-2085

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Induction of a Massive Endoplasmic Reticulum and Perinuclear Space Expansion by Expression of Lamin B Receptor Mutants and the Related Sterol Reductases TM7SF2 and DHCR7
Zwerger M, Kolb T, Richter K, Karakesisoglou I, Herrmann H
Molecular Biology of the Cell 2010 January;21(2):354-368

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Membrane Insertion of the Pleckstrin Homology Domain Variable Loop 1 Is Critical for Dynamin-catalyzed Vesicle Scission
Ramachandran R, Pucadyil T, Liu Y, Acharya S, Leonard M, Lukiyanchuk V, Schmid S
Molecular Biology of the Cell 2009 November;20(22):4630-4639

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows positivity in nuclear membrane.

Sample type

HUMAN

Enhanced validation

Submitted by

55af80e3e0991

Enhanced method

Genetic validation

Main image

Experimental details

Confocal images of immunofluorescently stained human U-2 OS cells.The protein SUN1 is shown in green. The image to the left show cells transfected with control siRNA and the image to the right show cells where SUN1 has been downregulated with specific siRNA.

Sample type

U-2 OS cells

Primary Ab dilution

1:59

Secondary Ab

Secondary Ab

Secondary Ab dilution

1:800

Knockdown/Genetic Approaches Application

Immunocytochemistry

KD Reagent Type

siRNA

Downregulation

<50%

KD Reagent Provider

Thermo Fisher Scientific

KD Reagent Prod no

s23629

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Prod no 2

s23630

KD Reagent Page 2

KD Reagent Page 2

KD Reagent Prod no 3

s23631

KD Reagent Page 3

KD Reagent Page 3

Antibody Lot Number

R01574

Appendix

55b74f0b3932b.pdf

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Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta shows strong nuclear membrane positivity in trophoblastic cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skin shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon using Anti-SUN1 antibody HPA008346.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node using Anti-SUN1 antibody HPA008346.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human skin and pancreas tissues using Anti-SUN1 antibody. Corresponding SUN1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human colon, lymph node, pancreas and skin using Anti-SUN1 antibody HPA008346 (A) shows similar protein distribution across tissues to independent antibody HPA008461 (B).

Sample type

HUMAN

Antibody #2 product nr

HPA008461

Antibody provider

Atlas Antibodies

Independent Antibody Method

ANTIBODY

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