PA5-109888

antibody from Invitrogen Antibodies

Targeting: KLK4 EMSP, EMSP1, KLK-L1, PRSS17, PSTS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109888

Provider

Invitrogen Antibodies

Product name

KLK4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Sequence of this protein is as follows: QIINGEDCSP HSQPWQAALV MENELFCSGV LVHPQWVLSA AHCFQNSYTI GLGLHSLEAD QEPGSQMVEA SLSVRHPEYN RPLLANDLML IKLDESVSES DTIRSISIAS QCPTAGNSCL VSGWGLLANG RMPTVLQCVN VSVVSEEVCS KLYDPLYHPS MFCAGGGQDQ KDSCNGDSGG PLICNGYLQG LVSFGKAPCG QVGVPGVYTN LCKFTEWIEK TVQAS

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.49 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Crucial Roles of microRNA-16-5p and microRNA-27b-3p in Ameloblast Differentiation Through Regulation of Genes Associated With Amelogenesis Imperfecta.

Suzuki A, Yoshioka H, Liu T, Gull A, Singh N, Le T, Zhao Z, Iwata J
Frontiers in genetics 2022;13:788259

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Overexpression of miR-1306-5p, miR-3195, and miR-3914 Inhibits Ameloblast Differentiation through Suppression of Genes Associated with Human Amelogenesis Imperfecta.

Yoshioka H, Wang YY, Suzuki A, Shayegh M, Gajera MV, Zhao Z, Iwata J
International journal of molecular sciences 2021 Feb 23;22(4)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KLK4 in mouse heart, mouse brain, rat testis. Samples were incubated in polyclonal KLK4 antibody (Product # PA5-109888) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of KLK4 in MCF7 cells. Samples were incubated in polyclonal KLK4 antibody (Product # PA5-109888) using a dilution of 1:50, followed by DAPI.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Effects of overexpression of candidate miRNAs on ameloblast differentiation. ( a ) Scheme of the experimental timeline. ( b ) Gene expression of AMELX, AMTN, KLK4, and MMP20 after treatment with a mimic of control or candidate miRNAs in AM-1 cells. * p < 0.05; ** p < 0.01, *** p < 0.001. ( c ) Immunocytochemistry analysis for KLK4 (green) in AM-1 cells under the indicated conditions. The nuclei were counterstained with 4',6'-diamidino-2-phenylindole [DAPI (blue)]. Scale bar, 150 mum.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 4 Effects of overexpression of candidate miRNAs asssociated with amelogenesis imperfecta on ameloblast differentiation. (A) Schematic of the experiment. (B) Gene expression of the indicated genes after treatment with the mimic for the indicated miRNA in mHAT9d cells ( n = 6). ** p < 0.01; *** p < 0.001. (C) Immunoblotting for AMELX, KLK4, MMP20, and GAPDH (internal control) in mHAT9d cells under the indicated conditions. Graph shows the quantification of the immunoblotting. n = 3 per group. * p < 0.05; ** p < 0.01; *** p < 0.001. (D) ICC for AMELX in mHAT9d cells under the indicated conditions. Scale bar, 50 mum. Graph shows the quantification of images from four independent experiments. ** p < 0.01; *** p < 0.001.