PA5-27252

antibody from Invitrogen Antibodies

Targeting: KLK7 PRSS6, SCCE

Antibody data

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Validation data

Product number: PA5-27252 - Provider product page
Provider: Invitrogen Antibodies
Product name: Kallikrein 7 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Recommended positive controls: A431, human KLK7-transfected 293T cells.
Concentration: 0.31 mg/mL

KLK7 protein structure - PA5-27252 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of KLK7 using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of human KLK7-transfected 293T cells. Samples were loaded onto a 12% SDS-PAGE gel and probed with a KLK7 polyclonal antibody (Product # PA5-27252) at a dilution of 1:5000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Kallikrein 7 was performed by separating 50 µg of mouse tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Kallikrein 7 Polyclonal Antibody (Product # PA5-27252) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Kallikrein 7 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Kallikrein 7 Polyclonal Antibody (Product # PA5-27252) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Kallikrein 7 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Kallikrein 7 Polyclonal Antibody (Product # PA5-27252) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Kallikrein 7 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Kallikrein 7 Polyclonal Antibody (Product # PA5-27252) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.