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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-38058 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LRRC26 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A recommended positive control is human prostate tissue lysate.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Hydrogen Sulfide Relaxes Human Uterine Artery via Activating Smooth Muscle BK(Ca) Channels.
Li Y, Bai J, Yang YH, Hoshi N, Chen DB
Antioxidants (Basel, Switzerland) 2020 Nov 13;9(11)
Antioxidants (Basel, Switzerland) 2020 Nov 13;9(11)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LRRC26 in human prostate tissue lysate using a LRRC26 polyclonal antibody (Product # PA5-38058) at a dilution of 0.5 µg/mL in (A) the absence and (B) the presence of a blocking peptide.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of LRRC26 in human prostate tissue lysate with LRRC26 Polyclonal Antibody (Product # PA5-38058) at 0.5 µg/mL in (A) the absence and (B) the presence of blocking peptide.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of LRRC26 in Jurkat cell lysate with LRRC26 Polyclonal Antibody (Product # PA5-38058) at 0.5 µg/mL in (A) the absence and (B) the presence of blocking peptide.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of LRRC26 in human prostate tissue with LRRC26 Polyclonal Antibody (Product # PA5-38058) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of LRRC26 in human prostate tissue with LRRC26 Polyclonal Antibody (Product # PA5-38058) at 2.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of LRRC26 in human prostate tissue with LRRC26 Polyclonal Antibody (Product # PA5-38058) at 2.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 BK Ca channel expression in human uterine artery. ( A ) Expression of mRNAs of BK Ca subunits in human uterine artery (hUA, upper panel) and cultured primary hUA smooth muscle cells (hUASMC, lower panel). Steady-state mRNAs of alpha, beta1-4, and gamma1-4 subunits were detected by RT-PCR. The amplicons were sequencing confirmed. M, 100 bp DNA ladder. ( B ) beta1 and gamma1 proteins detected by immunoblotting in hUA from two women and primary hUASMC in three passages (P). ( C ) Localization of beta1 and gamma3 proteins by immunofluorescence microscopy. SMC; smooth muscle cells; EC; endothelial cells; L; lumen; NC: negative control; SV; small vessels. Graph summarized levels of EC and SMC beta1 and gamma3 proteins ( n = 3). * p < 0.05.
