MA5-27618

antibody from Invitrogen Antibodies

Targeting: SCN3B HSA243396

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27618

Provider

Invitrogen Antibodies

Product name

SCN3B Monoclonal Antibody (N396/29)

Antibody type

Monoclonal

Antigen

Other

Description

A 1:100 dilution of MA5-27618 was sufficient for detection of NavBeta3 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 40kDa. Does not cross-reat with NavBeta1, Navbeta2, or Navbeta4. This antibody was formerly sold as clone S396-29.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

N396/29

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SCN3B in mouse brain with 20 µg of sample. The sample was incubated with SCN3B monoclonal antibody (Product # MA5-27618) using a dilution of 1:1000 (16 hours at 4°C), and followed by Goat Anti-Mouse (1 hour at RT) at a dilution of 1:200 and ECL development (5 min at RT). Samples were arranged as follows: Lane 1: MW Ladder, Lane 2: Mouse Brain.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SCN3B in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with SCN3B monoclonal antibody (Product # MA5-27618) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Nav beta 3 Antibody, D) Merged image. Magnification: 60x.