14-9896-82

antibody from Invitrogen Antibodies
Targeting: MSI1
Provider product page for 14-9896-82
Western blot
Immunocytochemistry
Immunohistochemistry
Other assay

Antibody data

Product number
14-9896-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
Musashi-1 Monoclonal Antibody (14H1), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This 14H1 monoclonal antibody recognizes human Musashi-1 (MSI-1), a 39 kDa RNA binding protein. Musashi-1 is expressed in CNS stem and progenitor cells with decreased expression in differentiating cells. Musashi-1 functions as a translational repressor, directly regulating its target proteins, Numb and p21 (CIP1). Musashi-1 has been shown to be expressed in stem cells in a variety of other tissues such as the gut, stomach, mammary gland, and hair follicles. In addition to normal tissue expression, Musashi-1 expression has been identified in cells from several tumor types, mainly pulmonary and adenocarcinoma, as well as large and small cell carcinomas. Recently, expression of Musashi-1 in ischemic lesions following stroke provides evidence for neurogenesis as well as the role of Musashi-1 in regulating apoptosis. Musashi-1 is highly conserved and the 14H1 antibody also recognizes mouse and rat Musashi-1. Applications Reported: This 14H1 antibody has been reported for use in immunoblotting (WB), immunohistology staining of paraffin embedded tissue sections, and immunocytochemistry. Applications Tested: This 14H1 antibody has been tested by immunofluorescent staining of paraformaldehyde fixed and permeabilized cells. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse, Rat
Host
Rat
Isotype
IgG
Antibody clone number
14H1
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4°C
MSI1 protein structure - 14-9896-82 shown in red.