PA5-96187

antibody from Invitrogen Antibodies

Targeting: MSI1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96187

Provider

Invitrogen Antibodies

Product name

MSI1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: SRVMPYGMDA FMLGIGMLGY PGFQATTYAS RSYTGLAPGY TYQFPEFRVE RTPLPSAPVL PELTAIPLTA YGPMAAAAAA AAVVRGTGSH PWTMAPPPGS TPSRTGGFLG TTSPGPMAEL YGAANQDSGV SSYISAASPA PSTGFGHSLG GPLIATAFTN GYH Positive Samples: A-549; Cellular Location: Cytoplasm, Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.62 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of A-549 cells, using MSI1 Polyclonal antibody (Product # PA5-96187) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MSI1 in extracts of various cell lines using MSI1 Polyclonal Antibody (Product # PA5-96187) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of MSI1 was performed in U2OS cells using MSI1 Polyclonal Antibody (Product # PA5-96187).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MSI1 in paraffin-embedded rat lung using MSI1 Polyclonal Antibody (Product # PA5-96187) at a dilution of 1:200.