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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- H00003382-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003382-M01, RRID:AB_606426
- Product name
- ICA1 monoclonal antibody (M01), clone 6G11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant ICA1.
- Antigen sequence
MSGHKCSYPWDLQDRYAQDKSVVNKMQQKYWETKQ
AFIKATGKKEDEHVVASDADLDAKLELFHSIQRTC
LDLSKAIVLYQKRICFLSQEENELGKFLRSQGFQD
KTRAG- Isotype
- IgG
- Antibody clone number
- 6G11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ICA1 expression in transfected 293T cell line by ICA1 monoclonal antibody (M01), clone 6G11.Lane 1: ICA1 transfected lysate (Predicted MW: 54.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ICA1 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
