MA5-29377

antibody from Invitrogen Antibodies

Targeting: KLK11 PRSS20, TLSP

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29377

Provider

Invitrogen Antibodies

Product name

Kallikrein 11 Recombinant Rabbit Monoclonal Antibody (121)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. This antibody has specificity for Human KLK-11/Kallikrein 11.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

121

Vial size

100 µL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Kallikrein 11 Recombinant Rabbit Monoclonal Antibody (121) (Product # MA5-29377) at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 42 kDa.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Kallikrein 11 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate 1 µL with Kallikrein 11 Recombinant Rabbit Monoclonal Antibody (121) (Product # MA5-29377) and 15 µL of 50 % Protein G agarose. Primary antibody: Kallikrein 11 Recombinant Rabbit Monoclonal Antibody (121), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 49 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Kallikrein 11 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate 1 µL with Kallikrein 11 Recombinant Rabbit Monoclonal Antibody (121) (Product # MA5-29377) and 15 µL of 50 % Protein G agarose. Primary antibody: Kallikrein 11 Recombinant Rabbit Monoclonal Antibody (121), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 49 kDa.