MA5-29377

antibody from Invitrogen Antibodies

Targeting: KLK11 PRSS20, TLSP

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29377

Provider

Invitrogen Antibodies

Product name

Kallikrein 11 Recombinant Rabbit Monoclonal Antibody (121)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Antibody clone number

121

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Kallikrein 11 in Lane A: A549 Whole Cell Lysate (30 µg). Samples were probed using a Kallikrein 11 Monoclonal Antibody (Product # MA5-29377) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:31 kDa. Observed band size:42 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Kallikrein 11 Recombinant Rabbit Monoclonal Antibody (121) (Product # MA5-29377) at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 42 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Kallikrein 11 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate 1 µL with Kallikrein 11 Recombinant Rabbit Monoclonal Antibody (121) (Product # MA5-29377) and 15 µL of 50 % Protein G agarose. Primary antibody: Kallikrein 11 Recombinant Rabbit Monoclonal Antibody (121), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 49 kDa.