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Learn11674-1-AP
antibody from Invitrogen Antibodies
Targeting: PDLIM1
CLIM1, CLP-36, CLP36, hCLIM1
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 11674-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDLIM1/CLP36 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.6 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human lung tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human ileum tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody at dilution of 1:600 incubated at 4 degree celsius over night.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP ( PDLIM1/CLP36 antibody at dilution of 1:600 incubated at 4 degree celsius over night.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using PDLIM1 antibody 11674-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer using 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:50 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 MCF-7 cells were stained with 0.2ug PDLIM1/CLP36 antibody (11674-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PDLIM1/CLP36 (IP:11674-1-AP, 3ug; Detection:11674-1-AP 1:600) with mouse heart tissue lysate 4000ug.
