16936-1-AP

antibody from Proteintech Group

Targeting: RNF10 KIAA0262, RIE2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16936-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16936-1-AP, RRID:AB_2181846

Product name

RNF10 antibody

Antibody type

Polyclonal

Description

KD/KO validated RNF10 antibody (Cat. #16936-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

RIOK3 mediates the degradation of 40S ribosomes.

Huang Z, Diehl FF, Wang M, Li Y, Song A, Chen FX, Rosa-Mercado NA, Beckmann R, Green R, Cheng J
Molecular cell 2025 Feb 20;85(4):802-814.e12

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The integrated stress response regulates 18S nonfunctional rRNA decay in mammals.

Coria AR, Shah A, Shafieinouri M, Taylor SJ, Orgebin E, Guiblet W, Miller JT, Sharma IM, Wu CC
Molecular cell 2025 Feb 20;85(4):787-801.e8

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E3 ubiquitin ligase RNF10 promotes dissociation of stalled ribosomes and responds to ribosomal subunit imbalance.

Lehmann JA, Lindner D, Sung HM, Stoecklin G
Nature communications 2024 Nov 28;15(1):10350

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RNF4 sustains Myc-driven tumorigenesis by facilitating DNA replication.

Her J, Zheng H, Bunting SF
The Journal of clinical investigation 2024 Mar 26;134(10)

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Gut microbial metabolite facilitates colorectal cancer development via ferroptosis inhibition.

Cui W, Guo M, Liu D, Xiao P, Yang C, Huang H, Liang C, Yang Y, Fu X, Zhang Y, Liu J, Shi S, Cong J, Han Z, Xu Y, Du L, Yin C, Zhang Y, Sun J, Gu W, Chai R, Zhu S, Chu B
Nature cell biology 2024 Jan;26(1):124-137

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The E3 ubiquitin ligase RNF10 modifies 40S ribosomal subunits of ribosomes compromised in translation.

Garzia A, Meyer C, Tuschl T
Cell reports 2021 Aug 3;36(5):109468

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The Synaptonuclear Messenger RNF10 Acts as an Architect of Neuronal Morphology.

Carrano N, Samaddar T, Brunialti E, Franchini L, Marcello E, Ciana P, Mauceri D, Di Luca M, Gardoni F
Molecular neurobiology 2019 Nov;56(11):7583-7593

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Ring finger protein 10 is a novel synaptonuclear messenger encoding activation of NMDA receptors in hippocampus.

Dinamarca MC, Guzzetti F, Karpova A, Lim D, Mitro N, Musardo S, Mellone M, Marcello E, Stanic J, Samaddar T, Burguière A, Caldarelli A, Genazzani AA, Perroy J, Fagni L, Canonico PL, Kreutz MR, Gardoni F, Di Luca M
eLife 2016 Mar 15;5:e12430

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 16936-1-AP(RNF10 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using RNF10 antibody 16936-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-RNF10 (IP:16936-1-AP, 5ug; Detection:16936-1-AP 1:500) with PC-3 cells lysate 2200ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 16936-1-AP(RNF10 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 16936-1-AP(RNF10 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue