Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 16936-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#16936-1-AP, RRID:AB_2181846
- Product name
- RNF10 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated RNF10 antibody (Cat. #16936-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references The E3 ubiquitin ligase RNF10 modifies 40S ribosomal subunits of ribosomes compromised in translation.
The Synaptonuclear Messenger RNF10 Acts as an Architect of Neuronal Morphology.
Ring finger protein 10 is a novel synaptonuclear messenger encoding activation of NMDA receptors in hippocampus.
Garzia A, Meyer C, Tuschl T
Cell reports 2021 Aug 3;36(5):109468
Cell reports 2021 Aug 3;36(5):109468
The Synaptonuclear Messenger RNF10 Acts as an Architect of Neuronal Morphology.
Carrano N, Samaddar T, Brunialti E, Franchini L, Marcello E, Ciana P, Mauceri D, Di Luca M, Gardoni F
Molecular neurobiology 2019 Nov;56(11):7583-7593
Molecular neurobiology 2019 Nov;56(11):7583-7593
Ring finger protein 10 is a novel synaptonuclear messenger encoding activation of NMDA receptors in hippocampus.
Dinamarca MC, Guzzetti F, Karpova A, Lim D, Mitro N, Musardo S, Mellone M, Marcello E, Stanic J, Samaddar T, Burguière A, Caldarelli A, Genazzani AA, Perroy J, Fagni L, Canonico PL, Kreutz MR, Gardoni F, Di Luca M
eLife 2016 Mar 15;5:e12430
eLife 2016 Mar 15;5:e12430
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 16936-1-AP(RNF10 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using RNF10 antibody 16936-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-RNF10 (IP:16936-1-AP, 5ug; Detection:16936-1-AP 1:500) with PC-3 cells lysate 2200ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human brain using 16936-1-AP(RNF10 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human brain using 16936-1-AP(RNF10 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue