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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 16936-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNF10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LPEACDDLE LADDNLKEGT ICTESSQQEP ITKSGFTRLS SSPCYYFYQA EDGQHMFLHP VNVRCLVREY GSLERSPEKI SATVVEIAGY SMSEDVRQRH RYLSHLPLTC EFSICELALQ PPVVSKETLE MFSDDIEKRK RQRQKKAREE RRRERRIEIE ENKKQGKYPE VHIPLENLQQ FPAFNSYTCS SDSALGPTST EGHGALSISP LSRSPGSHAD FLLTPLSPTA SQGSPSFCVG SLEEDSPFPS FAQMLRVGKA KADVWPKTAP KKDENSLVPP APVDSDGESD NSDRVPVPSF QNSFSQAIEA AFMKLDTPAT SDPLSEEKGG KKRKKQKQKL LFSTSVVHTK (463-811 aa encoded by BC016622)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.29 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using RNF10 antibody 16936-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 16936-1-AP (RNF10 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 16936-1-AP (RNF10 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RNF10 (IP:16936-1-AP, 5ug; Detection:16936-1-AP 1:500) with PC-3 cells lysate 2200ug.
