AVR-002-25UL

antibody from Invitrogen Antibodies

Targeting: VIPR2 VPAC2, VPAC2R

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AVR-002-25UL

Provider

Invitrogen Antibodies

Product name

VPAC2 (VIPR2) (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5), and human Malme-3M melanoma cell lysate (lanes 3 and 6): - 1-3. Anti-VPAC2 (VIPR2) (extracellular) Antibody (#AVR-002), (1:600).4-6. Anti-VPAC2 (VIPR2) (extracellular) Antibody , preincubated with VPAC2/VIPR2 (extracellular) Blocking Peptide (#BLP-VR002).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5), and human Malme-3M melanoma cell lysate (lanes 3 and 6): - 1-3. Anti-VPAC2 (VIPR2) (extracellular) Antibody (#AVR-002), (1:600).4-6. Anti-VPAC2 (VIPR2) (extracellular) Antibody , preincubated with VPAC2/VIPR2 (extracellular) Blocking Peptide (#BLP-VR002).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of VPAC2 in rat PC12 cells - Cell surface detection of VPAC2 in live intact rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells with Anti-VPAC2 (VIPR2) (extracellular) Antibody (#AVR-002), (1:50), followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Live view of the cells. C. Merge of A and B.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of VPAC2 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-APC. (green) Cells + Anti-VPAC2 (VIPR2) (extracellular) Antibody (#AVR-002), (2.5μg) + goat- Anti-rabbit-APC.