11648-2-AP

antibody from Proteintech Group

Targeting: YWHAE FLJ45465

Provider product page for 11648-2-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [25]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [1]

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Product number: 11648-2-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#11648-2-AP, RRID:AB_2217787
Product name: 14-3-3 Epsilon antibody
Antibody type: Polyclonal
Description: KD/KO validated 14-3-3 Epsilon antibody (Cat. #11648-2-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

YWHAE protein structure - 11648-2-AP shown in red.

Submitted references Nuclear receptor subfamily 2 group F member 2 transcriptionally activates 14-3-3 epsilon to promote diffuse large B-cell lymphoma progression.

Yu L, Wang Y, Li T, He X, Wang W
Cytotechnology 2026 Jun;78(3):96

Liquid-liquid phase separation of EphB4 drives pulmonary hypertension via YAP activation.

Liu JY, Chen J, Chen QY, Li HB, Gui LX, Zhou J, Lin MJ
Cell reports 2026 Feb 24;45(2):116992

Multi-omics integration reveals YWHAE as a key mediator of ferroptosis in ARDS.

Cui H, Huang X
Functional & integrative genomics 2025 Apr 22;25(1):94

The African swine fever virus MGF360-16R protein functions as a mitochondrial-dependent apoptosis inducer by competing with BAX to bind to the HSP60 protein.

Xiang Z, Xu Z, Weng W, Wang H, Wu J, Jiang F, Qu Y, Li Q, Gao P, Zhou L, Ge X, Guo X, Han J, Zhang Y, Yang H
Journal of virology 2025 Apr 15;99(4):e0140124

AGR2-mediated unconventional secretion of 14-3-3ε and α-actinin-4, responsive to ER stress and autophagy, drives chemotaxis in canine mammary tumor cells.

Yuan SH, Wu CC, Wang YC, Chan XY, Chu HW, Yang Y, Liu HP
Cellular & molecular biology letters 2024 May 31;29(1):84

circSORBS1 inhibits lung cancer progression by sponging miR-6779-5p and directly binding RUFY3 mRNA.

Xu H, Zheng Y, Wu J, Zhang R, Zhao Q, Chen S, Peng W, Cai D, Gao Y, Chen X, Li D, Yuan S, Li G, Nan A
Journal of translational medicine 2024 Jun 24;22(1):590

Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity.

Luo Q, Pan Y, Fu Q, Zhang X, Zhou S, Yu P, Tian H, Liu P, Chen S, Zhang H, Qin T
Cell biology and toxicology 2023 Oct;39(5):2069-2087

Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation.

Liu J, Zhao C, Xiao X, Li A, Liu Y, Zhao J, Fan L, Liang Z, Pang W, Yao W, Li W, Zhou J
Nature communications 2023 Oct 13;14(1):6457

Stabilization of 14-3-3 protein-protein interactions with Fusicoccin-A decreases alpha-synuclein dependent cell-autonomous death in neuronal and mouse models.

Vinueza-Gavilanes R, Bravo-González JJ, Basurco L, Boncristiani C, Fernández-Irigoyen J, Santamaría E, Marcilla I, Pérez-Mediavilla A, Luquin MR, Vales A, González-Aseguinolaza G, Aymerich MS, Aragón T, Arrasate M
Neurobiology of disease 2023 Jul;183:106166

LncRNA Gm16638-201 Inhibits the 14-3-3Ɛ Pathway in the Murine Prefrontal Cortex to Induce Depressive Behaviors.

Zhang T, Wang WL, Liu TJ, Lu S, Bian YC, Xiao R, Zhang CL
Biological & pharmaceutical bulletin 2022;45(11):1616-1626

AKT2 reduces IFNβ1 production to modulate antiviral responses and systemic lupus erythematosus.

Zheng X, Xiao J, Jiang Q, Zheng L, Liu C, Dong C, Zheng Y, Ni P, Zhang C, Zhang F, Zhong R, Ding H, Wang Q, Qiu Y, Gao M, Ding J, Shen N, Wei B, Wang H
The EMBO journal 2022 Mar 15;41(6):e108016

Melatonin Alleviates Hyperglycemia-Induced Cardiomyocyte Apoptosis via Regulation of Long Non-Coding RNA H19/miR-29c/MAPK Axis in Diabetic Cardiomyopathy.

Tang H, Zhong H, Liu W, Wang Y, Wang Y, Wang L, Tang S, Zhu H
Pharmaceuticals (Basel, Switzerland) 2022 Jul 2;15(7)

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.

Michalik S, Siegerist F, Palankar R, Franzke K, Schindler M, Reder A, Seifert U, Cammann C, Wesche J, Steil L, Hentschker C, Gesell-Salazar M, Reisinger E, Beer M, Endlich N, Greinacher A, Völker U
Haematologica 2022 Apr 1;107(4):947-957

A Novel Heptapeptide, GPPGPAG Transfers to the Brain, and Ameliorates Memory Dysfunction and Dendritic Atrophy in Alzheimer's Disease Model Mice.

Tohda C, Kogure C, Nomoto K, de Toledo A, Yang X, Hirano E
Frontiers in pharmacology 2021;12:680652

Role of the Chaperone Protein 14-3-3ε in the Regulation of Influenza A Virus-Activated Beta Interferon.

Tam EH, Liu YC, Woung CH, Liu HM, Wu GH, Wu CC, Kuo RL
Journal of virology 2021 Sep 27;95(20):e0023121

Akt kinase LANCL2 functions as a key driver in EGFR-mutant lung adenocarcinoma tumorigenesis.

Lou Y, Xu J, Zhang Y, Zhang W, Zhang X, Gu P, Zhong H, Wang H, Lu J, Han B
Cell death & disease 2021 Feb 10;12(2):170

Germacrone Regulates HBXIP-Mediated Cell Cycle, Apoptosis and Promotes the Formation of Autophagosomes to Inhibit the Proliferation of Gastric Cancer Cells.

Fang X, Tan T, Gao B, Zhao Y, Liu T, Xia Q
Frontiers in oncology 2020;10:537322

1,3-Dicaffeoylquinic acid targeting 14-3-3 tau suppresses human breast cancer cell proliferation and metastasis through IL6/JAK2/PI3K pathway.

Zhou Y, Fu X, Guan Y, Gong M, He K, Huang B
Biochemical pharmacology 2020 Feb;172:113752

Interactome of the Autoimmune Risk Protein ANKRD55.

Ugidos N, Mena J, Baquero S, Alloza I, Azkargorta M, Elortza F, Vandenbroeck K
Frontiers in immunology 2019;10:2067

Zika Virus NS3 Mimics a Cellular 14-3-3-Binding Motif to Antagonize RIG-I- and MDA5-Mediated Innate Immunity.

Riedl W, Acharya D, Lee JH, Liu G, Serman T, Chiang C, Chan YK, Diamond MS, Gack MU
Cell host & microbe 2019 Oct 9;26(4):493-503.e6

14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling.

Song J, Zhang X, Liao Z, Liang H, Chu L, Dong W, Zhang X, Ge Q, Liu Q, Fan P, Zhang Z, Zhang B
Journal of experimental & clinical cancer research : CR 2019 Jan 3;38(1):3

CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer.

Li H, Jin X, Liu B, Zhang P, Chen W, Li Q
BMC cancer 2019 Aug 22;19(1):826

14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells.

Lu K, Rui G, Liu F, Yang L, Deng X, Shi S, Li Q
Oncology letters 2018 Jan;15(1):338-346

Specific Alterations in Astrocyte Properties via the GluA2-GAPDH Complex Associated with Multiple Sclerosis.

Lee FHF, Zhang H, Jiang A, Zai CC, Liu F
Scientific reports 2018 Aug 27;8(1):12856

A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity.

Chan YK, Gack MU
Nature immunology 2016 May;17(5):523-30

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: A375 cells were subjected to SDS PAGE followed by western blot with 11648-2-AP(14-3-3E antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using YWHAE antibody 11648-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-14-3-3E (IP:11648-2-AP, 3ug; Detection:11648-2-AP 1:1000) with A375 cells lysate 6000ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human gliomas using 11648-2-AP(14-3-3E antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue