11648-2-AP

antibody from Proteintech Group

Targeting: YWHAE FLJ45465

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

11648-2-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#11648-2-AP, RRID:AB_2217787

Product name

14-3-3 Epsilon antibody

Antibody type

Polyclonal

Description

KD/KO validated 14-3-3 Epsilon antibody (Cat. #11648-2-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

circSORBS1 inhibits lung cancer progression by sponging miR-6779-5p and directly binding RUFY3 mRNA.

Xu H, Zheng Y, Wu J, Zhang R, Zhao Q, Chen S, Peng W, Cai D, Gao Y, Chen X, Li D, Yuan S, Li G, Nan A
Journal of translational medicine 2024 Jun 24;22(1):590

---

Immortalization-upregulated protein promotes pancreatic cancer progression by regulating NPM1/FHL1-mediated cell-cycle-checkpoint protein activity.

Luo Q, Pan Y, Fu Q, Zhang X, Zhou S, Yu P, Tian H, Liu P, Chen S, Zhang H, Qin T
Cell biology and toxicology 2023 Oct;39(5):2069-2087

---

Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation.

Liu J, Zhao C, Xiao X, Li A, Liu Y, Zhao J, Fan L, Liang Z, Pang W, Yao W, Li W, Zhou J
Nature communications 2023 Oct 13;14(1):6457

---

Stabilization of 14-3-3 protein-protein interactions with Fusicoccin-A decreases alpha-synuclein dependent cell-autonomous death in neuronal and mouse models.

Vinueza-Gavilanes R, Bravo-González JJ, Basurco L, Boncristiani C, Fernández-Irigoyen J, Santamaría E, Marcilla I, Pérez-Mediavilla A, Luquin MR, Vales A, González-Aseguinolaza G, Aymerich MS, Aragón T, Arrasate M
Neurobiology of disease 2023 Jul;183:106166

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LncRNA Gm16638-201 Inhibits the 14-3-3Ɛ Pathway in the Murine Prefrontal Cortex to Induce Depressive Behaviors.

Zhang T, Wang WL, Liu TJ, Lu S, Bian YC, Xiao R, Zhang CL
Biological & pharmaceutical bulletin 2022;45(11):1616-1626

---

AKT2 reduces IFNβ1 production to modulate antiviral responses and systemic lupus erythematosus.

Zheng X, Xiao J, Jiang Q, Zheng L, Liu C, Dong C, Zheng Y, Ni P, Zhang C, Zhang F, Zhong R, Ding H, Wang Q, Qiu Y, Gao M, Ding J, Shen N, Wei B, Wang H
The EMBO journal 2022 Mar 15;41(6):e108016

---

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.

Michalik S, Siegerist F, Palankar R, Franzke K, Schindler M, Reder A, Seifert U, Cammann C, Wesche J, Steil L, Hentschker C, Gesell-Salazar M, Reisinger E, Beer M, Endlich N, Greinacher A, Völker U
Haematologica 2022 Apr 1;107(4):947-957

---

A Novel Heptapeptide, GPPGPAG Transfers to the Brain, and Ameliorates Memory Dysfunction and Dendritic Atrophy in Alzheimer's Disease Model Mice.

Tohda C, Kogure C, Nomoto K, de Toledo A, Yang X, Hirano E
Frontiers in pharmacology 2021;12:680652

---

Role of the Chaperone Protein 14-3-3ε in the Regulation of Influenza A Virus-Activated Beta Interferon.

Tam EH, Liu YC, Woung CH, Liu HM, Wu GH, Wu CC, Kuo RL
Journal of virology 2021 Sep 27;95(20):e0023121

---

Akt kinase LANCL2 functions as a key driver in EGFR-mutant lung adenocarcinoma tumorigenesis.

Lou Y, Xu J, Zhang Y, Zhang W, Zhang X, Gu P, Zhong H, Wang H, Lu J, Han B
Cell death & disease 2021 Feb 10;12(2):170

---

1,3-Dicaffeoylquinic acid targeting 14-3-3 tau suppresses human breast cancer cell proliferation and metastasis through IL6/JAK2/PI3K pathway.

Zhou Y, Fu X, Guan Y, Gong M, He K, Huang B
Biochemical pharmacology 2020 Feb;172:113752

---

Interactome of the Autoimmune Risk Protein ANKRD55.

Ugidos N, Mena J, Baquero S, Alloza I, Azkargorta M, Elortza F, Vandenbroeck K
Frontiers in immunology 2019;10:2067

---

Zika Virus NS3 Mimics a Cellular 14-3-3-Binding Motif to Antagonize RIG-I- and MDA5-Mediated Innate Immunity.

Riedl W, Acharya D, Lee JH, Liu G, Serman T, Chiang C, Chan YK, Diamond MS, Gack MU
Cell host & microbe 2019 Oct 9;26(4):493-503.e6

---

14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling.

Song J, Zhang X, Liao Z, Liang H, Chu L, Dong W, Zhang X, Ge Q, Liu Q, Fan P, Zhang Z, Zhang B
Journal of experimental & clinical cancer research : CR 2019 Jan 3;38(1):3

---

CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer.

Li H, Jin X, Liu B, Zhang P, Chen W, Li Q
BMC cancer 2019 Aug 22;19(1):826

---

14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells.

Lu K, Rui G, Liu F, Yang L, Deng X, Shi S, Li Q
Oncology letters 2018 Jan;15(1):338-346

---

Specific Alterations in Astrocyte Properties via the GluA2-GAPDH Complex Associated with Multiple Sclerosis.

Lee FHF, Zhang H, Jiang A, Zai CC, Liu F
Scientific reports 2018 Aug 27;8(1):12856

---

A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity.

Chan YK, Gack MU
Nature immunology 2016 May;17(5):523-30

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 11648-2-AP(14-3-3E antibody) at dilution of 1:500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using YWHAE antibody 11648-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-14-3-3E (IP:11648-2-AP, 3ug; Detection:11648-2-AP 1:1000) with A375 cells lysate 6000ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human gliomas using 11648-2-AP(14-3-3E antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue