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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX109090 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109090, RRID:AB_1952629
- Product name
- 14-3-3 epsilon antibody [N1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Secretome protein signature of human pancreatic cancer stem-like cells.
Brandi J, Dalla Pozza E, Dando I, Biondani G, Robotti E, Jenkins R, Elliott V, Park K, Marengo E, Costello E, Scarpa A, Palmieri M, Cecconi D
Journal of proteomics 2016 Mar 16;136:1-12
Journal of proteomics 2016 Mar 16;136:1-12
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ?g of whole cell lysate) A: mouse brain 12% SDS PAGE GTX109090 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate)A: H1299B: HeLa S312% SDS PAGEGTX109090 diluted at 1:5000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 epsilon antibody [N1C3] (GTX109090) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 epsilon(GTX109090) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- 14-3-3 epsilon antibody [N1C3] immunoprecipitates YWHAE protein in IP experiments.IP samples: HeLa whole cell extractA. 40 £gg HeLa whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of YWHAE protein by 4 £gg 14-3-3 epsilon antibody [N1C3] (GTX109090)12 % SDS-PAGEThe immunoprecipitated YWHAE protein was detected by 14-3-3 epsilon antibody [N1C3] (GTX109090) diluted at 1:1000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft , using 14-3-3 epsilon(GTX109090) antibody at 1:500 dilution.
