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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA321782 - Provider product page
- Provider
- OriGene
- Product name
- Anti-YWHAE Rabbit Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- Anti-YWHAE Rabbit Polyclonal Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- YWHAE
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- 4.4mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Predicted band size: 29 kDa . Positive control: Human brain malignant glioma and laryngocarcinoma tissue . Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lane1: Human brain malignant glioma tissue lysate Lane2: Human laryngocarcinoma tissue lysate Lysates: 40 ug per lane Primary antibody: 1/2200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Predicted cell location: Cytoplasm, Nucleus. Positive control: Human colon cancer tissue. Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using YWHAE antibody at dilution 1/100, on the right is treated with the synthetic peptide. (Original magnification:x200)
- Validation comment
- IHC
