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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ5785 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- 14-3-3 epsilon Antibody / YWHAE
- Antibody type
- Polyclonal
- Description
- This highly specific 14-3-3 epsilon antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Immunofluorescence/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the 14-3-3 epsilon antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) Jurkat, 3) HepG2, 4) SH-SY5Y, 5) HEK293, 6) SW620, 7) A549, 8) Raji, 9) rat brain, 10) mouse brain, 11) mouse NIH 3T3 and 12) mouse RAW264.7 lysate with 14-3-3 epsilon antibody. Predicted molecular weight ~28 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human MCF7 cells with 14-3-3 epsilon antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat brain with 14-3-3 epsilon antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human A549 cells with 14-3-3 epsilon antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= 14-3-3 epsilon antibody.
