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- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-28937 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 14-3-3 epsilon Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain. Predicted reactivity: Mouse (100%), Rat (98%), Xenopus laevis (96%), Chicken (100%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.91 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 14-3-3 epsilon in methanol-fixed HeLa cells using a 14-3-3 epsilon polyclonal antibody (Product # PA5-28937) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of 14-3-3 epsilon was performed using Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with 14-3-3 epsilon Polyclonal Antibody (Product # PA5-28937) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A27034, 1:2000 dilution) for 45 minutes at room temperature (Panel a: green) in HeLa cells. Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of Hep G2 cells showing nuclear and cytoplasmic localization for 14-3-3 epsilon. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of 14-3-3 epsilon was performed using Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with 14-3-3 epsilon Polyclonal Antibody (Product # PA5-28937) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A27034, 1:2000 dilution) for 45 minutes at room temperature (Panel a: green) in HeLa cells. Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of Hep G2 cells showing nuclear and cytoplasmic localization for 14-3-3 epsilon. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 14-3-3 epsilon Polyclonal Antibody immunoprecipitates YWHAE protein in IP experiments. IP samples: HeLa whole cell extract. A. 40 µg HeLa whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of YWHAE protein by 4 µg 14-3-3 epsilon Polyclonal Antibody (Product # PA5-28937). 12 % SDS-PAGE. The immunoprecipitated YWHAE protein was detected by 14-3-3 epsilon Polyclonal Antibody (Product # PA5-28937) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft , using 14-3-3 epsilon (Product # PA5-28937) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 14-3-3 epsilon Polyclonal Antibody immunoprecipitates YWHAE protein in IP experiments. IP samples: HeLa whole cell extract. A. 40 µg HeLa whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of YWHAE protein by 4 µg 14-3-3 epsilon Polyclonal Antibody (Product # PA5-28937). 12 % SDS-PAGE. The immunoprecipitated YWHAE protein was detected by 14-3-3 epsilon Polyclonal Antibody (Product # PA5-28937) diluted at 1:1,000.
