Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM09042PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM09042PU-S, RRID:AB_1609700
- Product name
- anti 14-3-3 protein epsilon
- Antibody type
- Monoclonal
- Antigen
- Recombinant Human 14-3-3 epsilon (1-255 aa) purified from E. coli.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5A5
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis: The extracts of mouse brain (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 epsilon (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- The extracts of mouse brain (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 epsilon (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry: AM09042PU-N YWHAE antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry: AM09042PU-N YWHAE antibody staining of Formalin-Fixed, Paraffin-Embedded Human Lung at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
