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- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- 16418-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#16418-1-AP, RRID:AB_2276536
- Product name
- NELFB antibody
- Antibody type
- Polyclonal
- Description
- NELFB antibody (Cat. #16418-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis.
An essential signaling function of cytoplasmic NELFB is independent of RNA polymerase II pausing.
RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis.
ERK-mediated NELF-A phosphorylation promotes transcription elongation of immediate-early genes by releasing promoter-proximal pausing of RNA polymerase II.
The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.
High constitutive activity of a broad panel of housekeeping and tissue-specific cis-regulatory elements depends on a subset of ETS proteins.
Kaye EG, Basavaraju K, Nelson GM, Zomer HD, Roy D, Joseph II, Rajabi-Toustani R, Qiao H, Adelman K, Reddi PP
Nature communications 2024 Jan 29;15(1):848
Nature communications 2024 Jan 29;15(1):848
An essential signaling function of cytoplasmic NELFB is independent of RNA polymerase II pausing.
Pan H, Cheng X, RodrÃguez PFG, Zhang X, Chung I, Jin VX, Li W, Hu Y, Li R
The Journal of biological chemistry 2023 Nov;299(11):105259
The Journal of biological chemistry 2023 Nov;299(11):105259
RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis.
Kaye EG, Nelson GM, Zomer HD, Roy D, Joseph II, Adelman K, Reddi PP
bioRxiv : the preprint server for biology 2023 May 9;
bioRxiv : the preprint server for biology 2023 May 9;
ERK-mediated NELF-A phosphorylation promotes transcription elongation of immediate-early genes by releasing promoter-proximal pausing of RNA polymerase II.
Ohe S, Kubota Y, Yamaguchi K, Takagi Y, Nashimoto J, Kozuka-Hata H, Oyama M, Furukawa Y, Takekawa M
Nature communications 2022 Dec 3;13(1):7476
Nature communications 2022 Dec 3;13(1):7476
The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.
Liu B, Xu Q, Wang Q, Feng S, Lai F, Wang P, Zheng F, Xiang Y, Wu J, Nie J, Qiu C, Xia W, Li L, Yu G, Lin Z, Xu K, Xiong Z, Kong F, Liu L, Huang C, Yu Y, Na J, Xie W
Nature 2020 Nov;587(7832):139-144
Nature 2020 Nov;587(7832):139-144
High constitutive activity of a broad panel of housekeeping and tissue-specific cis-regulatory elements depends on a subset of ETS proteins.
Curina A, Termanini A, Barozzi I, Prosperini E, Simonatto M, Polletti S, Silvola A, Soldi M, Austenaa L, Bonaldi T, Ghisletti S, Natoli G
Genes & development 2017 Feb 15;31(4):399-412
Genes & development 2017 Feb 15;31(4):399-412
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16418-1-AP(NELF-B antibody) at dilution of 1:800
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The NELFB antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human NELFB. This antibody recognizes human,mouse,rat antigen. The NELFB antibody has been validated for the following applications: ELISA, WB, IP analysis.
