PA5-28528

antibody from Invitrogen Antibodies

Targeting: SIGLEC7 CD328, p75/AIRM1, QA79, SIGLEC-7, SIGLEC19P, SIGLECP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28528

Provider

Invitrogen Antibodies

Product name

SIGLEC7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: K562, THP-1, HL-60, NCI-H929. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SIGLEC7 Polyclonal Antibody detects Siglec 7 protein by western blot analysis. A. 30 µg K562 whole cell lysate/extract. B. 30 µg THP-1 whole cell lysate/extract. C. 30 µg HL-60 whole cell lysate/extract. D. 30 µg NCI-H929 whole cell lysate/extract. 10% SDS-PAGE. SIGLEC7 Polyclonal Antibody (Product # PA5-28528) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Siglec-7/AIRM-1 in methanol-fixed A431 cells using a Siglec-7/AIRM-1 polyclonal antibody (Product # PA5-28528) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Siglec 7 (Product # PA5-28528) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.