NBP2-30699

antibody from Novus Biologicals

Targeting: DICER1 Dicer, HERNA, K12H4.8-LIKE, KIAA0928, MNG1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-30699

Provider

Novus Biologicals

Product name

Mouse Monoclonal Dicer Antibody

Antibody type

Monoclonal

Description

Protein A purified. Specificity of human Dicer antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Mouse

Antigen sequence

LSSAEKRKAK


Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Dicer Antibody (CL0378) [NBP2-30699] - Lane 1: Marker [kDa]. Lane 2: Human cell line HEK293T

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Dicer Antibody (CL0378) [NBP2-30699] - Analysis of extracts from RT-4 cells, transfected with either control siRNA (siRNA ctrl) or target specific siRNA probes (siRNA#1, siRNA#2), using Anti-DICER monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human breast cancer (ductal carcinoma) shows moderate cytoplasmic positivity in cancer cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human lung cancer (adenocarcinoma) shows strong cytoplasmic positivity in cancer cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human colorectal cancer (adenocarcinoma) shows cytoplasmic positivity in cancer cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human prostate shows cytoplasmic immunoreactivity in the glandular epithelium cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human uterus shows cytoplasmic immunoreactivity in the glandular epithelium cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human testis shows cytoplasmic positivity in the seminiferous tubules cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Dicer Antibody (CL0378) [NBP2-30699] - Staining of human cerebral cortex shows cytoplasmic positivity in the glial cells.