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LearnMA5-31353
antibody from Invitrogen Antibodies
Targeting: DICER1
Dicer, HERNA, K12H4.8-LIKE, KIAA0928, MNG1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [8]
- Other assay [2]
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- Product number
- MA5-31353 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Dicer Monoclonal Antibody (CL0378)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PTDADSAYCV LPLNVVNDSS TLDIDFKFME DIEKSEARIG IPSTKYTKET PFVFKLEDYQ DAVIIPRYRN FDQPHRFYVA DVYTDLTPLS KFPSPEYETF AEYYKTKYNL DLTNLNQPLL DVDHTSSRLN LLTPRHLNQK GKALPLSSAE KRKAKWESLQ NKQILVPELC AIHPIPASLW RKAVCLPSIL YRLH
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CL0378
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Small RNA expression and miRNA modification dynamics in human oocytes and early embryos.
MiRNA-103/107 in Primary High-Grade Serous Ovarian Cancer and Its Clinical Significance.
Paloviita P, Hydén-Granskog C, Yohannes DA, Paluoja P, Kere J, Tapanainen JS, Krjutškov K, Tuuri T, Võsa U, Vuoristo S
Genome research 2021 Aug;31(8):1474-1485
Genome research 2021 Aug;31(8):1474-1485
MiRNA-103/107 in Primary High-Grade Serous Ovarian Cancer and Its Clinical Significance.
Wilczynski M, Kielbik M, Senderowska D, Krawczyk T, Szymanska B, Klink M, Bieńkiewicz J, Romanowicz H, Frühauf F, Malinowski A
Cancers 2020 Sep 19;12(9)
Cancers 2020 Sep 19;12(9)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Dicer by a Dicer monoclonal antibody (Product # MA5-31353). Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Dicer by a Dicer monoclonal antibody (Product # MA5-31353). Lane 1: Marker [kDa] Lane 2: Human cell line HEK293T.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Dicer in A-431 cells using a Dicer monoclonal antibody (Product # MA5-31353). The analysis shows specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Dicer in human lung cancer (adenocacrinoma) using a Dicer monoclonal antibody (Product # MA5-31353). The analysis shows strong cytoplasmic positivity in cancer cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Dicer in human uterus using a Dicer monoclonal antibody (Product # MA5-31353). The analysis shows cytoplasmic immunoreactivity in the glandular epithelium cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Dicer in human testis using a Dicer monoclonal antibody (Product # MA5-31353). The analysis shows cytoplasmic positivity in the seminiferous tubules cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Dicer in human prostate using a Dicer monoclonal antibody (Product # MA5-31353). The analysis shows cytoplasmic immunoreactivity in the glandular epithelium cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Dicer in human cerebral cortex using a Dicer monoclonal antibody (Product # MA5-31353). The analysis shows cytoplasmic positivity in the glial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Dicer in human colorectal cancer (adenocarcinoma) using a Dicer monoclonal antibody (Product # MA5-31353). The analysis shows cytoplasmic positivity in cancer cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Dicer in human lung cancer (adenocacrinoma) using a Dicer monoclonal antibody (Product # MA5-31353). The analysis shows strong cytoplasmic positivity in cancer cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Dicer in human breast cancer (ductal carcinoma) using a Dicer monoclonal antibody (Product # MA5-31353). The analysis shows moderate cytoplasmic positivity in cancer cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 IHC (original magnification 40x) staining of ovarian cancer tissues: ( A ) Weak cytoplasmic positivity for DICER, ( B ) strong cytoplasmic positivity for DICER.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. Small RNA (sRNA) pathway component expression in human oocytes and embryos. ( A ) Human four-cell ( left ) and eight-cell ( right ) embryos immunostained with DGCR8 (green) and DICER1 (magenta) antibodies. Nuclei are counterstained with DAPI (blue). Overlay of a single representative z -plane and the corresponding z -planes are shown. Nucleus and bright field channels are on the right side of each overlay. Scale bar is 50 mum. Expression levels (RPKM) in human oocytes and preimplantation embryos (): ( B ) miRNA pathway gene, DGCR8 , DROSHA , DICER1 , and AGO1-4 ; ( C ) piRNA pathway gene, PLD6 , HENMT1 , and PIWIL1-4 . ( B , C ) Significant expression changes between consecutive developmental stages were assessed using pairwise, two-sided Wilcoxon rank-sum tests: (*) FDR < 0.05; (**) FDR < 0.01; (***) FDR < 0.001. The horizontal line in the box plot indicates the expression median.
