PA1-12565

antibody from Invitrogen Antibodies

Targeting: VSX2 CHX10, HOX10, RET1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-12565

Provider

Invitrogen Antibodies

Product name

CHX10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

By Western blot, this antibody detects a ~46 kDa band. A suggested positive control for this product is rat or mouse retinal tissue lysate.

Reactivity

Human, Mouse, Rat, Chicken/Avian

Host

Sheep

Isotype

IgG

Vial size

250 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Dose-dependent regulation of horizontal cell fate by Onecut family of transcription factors.

Kreplova M, Kuzelova A, Antosova B, Zilova L, Jägle H, Kozmik Z
PloS one 2020;15(8):e0237403

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Retina Organoid Transplants Develop Photoreceptors and Improve Visual Function in RCS Rats With RPE Dysfunction.

Lin B, McLelland BT, Aramant RB, Thomas BB, Nistor G, Keirstead HS, Seiler MJ
Investigative ophthalmology & visual science 2020 Sep 1;61(11):34

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CHX10 using a polyclonal antibody (Product # PA1-12565).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CHX10 using a polyclonal antibody (Product # PA1-12565).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CHX10 using a polyclonal antibody (Product # PA1-12565).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1. Characterization of retinal organoids. ( a ) Gene expression scatterplot (mean delta Ct) of d37-d70 retina organoids ( y -axis) versus d105-d145 human fetal retina ( x -axis). Highly co-expressed genes are shown in the center ( red ). The organoids ( n = 17) and fetal tissue ( n = 7) express similar levels of several important developmental genes. Differentially expressed genes are shown in the periphery ( grey ). The retina organoids contain all the major cellular subtypes responsible for support, synaptic integration, and phototransduction. These include glia, ganglion cells, bipolar, horizontal, amacrine, and Muller cells. RPL7 was used as a housekeeping gene. The mean delta Ct values are calculated from biological replicates. ( b ) Confocal microscopy for retinal ganglion cell marker Brn3b ( magenta ) (D51). Nuclei are grey (DAPI). ( c ) Synaptophysin staining of d 44 and d 51 retina organoids, showing mostly developing retinal ganglion cells and cones. ( d ) Confocal microscopy of d38 retinal organoids for MAP 2 ( green ), Chx10 ( gold ), OTX2 ( red ), and DAPI (nuclei, blue ). ( e ) Confocal microscopy of d38 organoids for MAP2 ( red ), Recoverin ( green ), Chx10 ( gold ), and DAPI ( blue ). The w hite arrows point to developing cone photoreceptors. Scale = 100 um ( b ); 50 um ( b , c ); 20 um ( d , e ). DAPI = 4'',6-Diamidino-2-phenylindole.